Coding

Part:BBa_K1371001

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-03)

KS-AT1


KS Part

The leading role of the KS part is Ketocaylsynthase, KS domain. Its last step is activation of propionyl groups. AT domains in Module 1 or Module 2 activate the propionyl groups in (2S)Methylmalonyl-CoA (details in Loading Part). Afterwards, KS domains combine the extended units anchored on the ACP domains belonging to the Modules which the KS domains belong to with the polyketide chains anchored on the ACP domains belonging to the former Modules (or Loadings). Then new polyketide chains form and are anchored the ACP domains belonging to the Modules which the KS domains belong to.

The function of KS domain is combining the propionyl group with acetyl group at the end of the polyketide chain by forming the C—C bond. In KS domain catalyzed reactions, polyketide chains are transferred from ACP domains belonging to the former Module (or Loading) onto KS domains, and then occur decarboxylic reaction with the extended units anchored on the ACP domains belonging to the Modules which the KS domains belong to. As a result, the polyketide chains are elongated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 685
    Illegal NgoMIV site found at 718
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 1900
    Illegal AgeI site found at 3
    Illegal AgeI site found at 349
    Illegal AgeI site found at 928
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2185
    Illegal SapI.rc site found at 1020


[edit]
Categories
Parameters
n/aKS-AT1